Article
GE2270A-resistant mutations in elongation factor Tu allow productive aminoacyl-tRNA binding to EF-Tu.GTP.GE2270A complexes.
Journal of molecular biology - 15 Dec 2000
Zuurmond A M, Martien de Graaf J, Olsthoorn-Tieleman L N, van Duyl B Y, Mörhle V G, Jurnak F, Mesters J R, Hilgenfeld R, Kraal B
Abstract excerpt
The antibiotic GE2270A prevents stable complex formation between elongation factor Tu (EF-Tu) and aminoacyl-tRNA (aatRNA). In Escherichia coli we characterized two mutant EF-Tu species with either G257S or G275A that lead to high GE2270A resistance in poly(Phe) synthesis, which at least partially explains the high resistance of EF-Tu1 from GE2270A producer Planobispora rosea to its own antibiotic. Both E. coli...
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