Article
The structural and functional basis for the kirromycin resistance of mutant EF-Tu species in Escherichia coli.
The EMBO journal - 17 Oct 1994
Mesters J R, Zeef L A, Hilgenfeld R, de Graaf J M, Kraal B, Bosch L
Abstract excerpt
A structural and functional understanding of resistance to the antibiotic kirromycin in Escherichia coli has been sought in order to shed new light on the functioning of the bacterial elongation factor Tu (EF-Tu), in particular its ability to act as a molecular switch. The mutant EF-Tu species G316D, A375T, A375V and Q124K, isolated by M13mp phage-mediated targeted mutagenesis, were studied. In this order the...
Topics
- Anti-Bacterial Agents
- Drug Resistance, Microbial
- Escherichia coli
- Guanosine Triphosphate
- Models, Molecular
- Mutation
- Peptide Elongation Factor Tu
- Protein Binding
- Pyridones
- RNA, Bacterial
- RNA, Transfer, Amino Acyl