Article
Probing the reactivity of the GTP- and GDP-bound conformations of elongation factor Tu in complex with the antibiotic GE2270 A.
The Journal of biological chemistry - 25 Nov 1993
Anborgh P H, Parmeggiani A
Abstract excerpt
The activity of Escherichia coli elongation factor Tu (EF-Tu) depends on its GTP- and GDP-bound conformations. In this work we have studied the influence of the antibiotic GE2270 A, a new EF-Tu-specific inhibitor of protein biosynthesis, on these two conformations with respect to the interaction with the various ligands and stability. One molecule of GE2270 A bound per EF-Tu is sufficient to block...
Topics
- Anti-Bacterial Agents
- Escherichia coli
- GTP Phosphohydrolase-Linked Elongation Factors
- Guanosine Diphosphate
- Guanosine Triphosphate
- Hot Temperature
- Mutation
- Peptide Elongation Factor Tu
- Peptides, Cyclic
- Protein Conformation