Article
Mutants of EF-Tu defective in binding aminoacyl-tRNA.
FEBS letters - 18 Mar 1996
Abdulkarim F, Ehrenberg M, Hughes D
Abstract excerpt
Five single amino acid substitution variants of EF-Tu from Salmonella typhimurium were tested for their ability to promote poly(U)-translation in vitro. The substitutions are Leu120 Gln, Gln124 Arg and Tyr160 (Asp or Asn or Cys). They were selected by their kirromycin resistant phenotypes and all substitutions are in domain I at the interface between domains I and III of the EF-Tu.GTP configuration. The different...
Topics
- Guanosine Triphosphate
- Kinetics
- Mutation
- Peptide Chain Elongation, Translational
- Peptide Elongation Factor Tu
- Poly U
- Protein Binding
- RNA, Transfer, Amino Acyl
- Ribosomes
- Salmonella typhimurium