Article
Subtilisin BPN' variants: increased hydrolytic activity on surface-bound substrates via decreased surface activity.
Biochemistry - 12 Mar 1996
Brode P F, Erwin C R, Rauch D S, Barnett B L, Armpriester J M, Wang E S, Rubingh D N
Abstract excerpt
Site-directed mutagenesis and random mutagenesis were used to produce variants of subtilisin BPN' (Bacillus amyloliquefaciens) protease with variable surface adsorption properties. Protease adsorption and peptide hydrolysis rate were measured for these variants using a model substrate consisting of a peptide covalently bound to a surface. While most variants adsorb at a level very similar to that of native BPN',...
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