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Article

Site-directed and random immobilization of subtilisin on functionalized membranes: activity determination in aqueous and organic media.

Biotechnology and bioengineering - 5 Dec 1998

Viswanath S, Wang J, Bachas L G, Butterfield D A, Bhattacharyya D

Abstract excerpt

Kinetic comparisons have been made between a randomly immobilized and a site-specifically immobilized subtilisin BPN' on microfiltration membranes of varying hydrophilicities in both aqueous and organic media. Site-directed mutagenesis was employed to introduce a single cysteine into the amino ac...

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PMID: 10099469

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 1 Sept 2026. Prepared record publication-10099469-2026-09-01.1. Manifest publication-graph-cohort-2026-09-01.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 1 Sept 2026.

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