Article
Structure of a switchable subtilisin complexed with a substrate and with the activator azide.
Biochemistry - 3 Nov 2009
Gallagher Travis, Ruan Biao, London Mariya, Bryan Molly A, Bryan Philip N
Abstract excerpt
An engineered variant of the protease subtilisin from Bacillus amyloliquefaciens, in which the D32A mutation renders the enzyme's activity dependent on the presence of certain small anions such as fluoride or azide, has been produced. This modified enzyme has applications as an azide or fluoride-triggered expression-purification tool. We report activity measurements showing that the enzyme is activated more than...
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