Article
Rational modification of enzyme catalysis by engineering surface charge.
Nature - 1 Jan 2000
Russell A J, Fersht A R
Abstract excerpt
Changing the surface charge of subtilisin by site-directed mutagenesis produces enzymes with significantly shifted pH-activity profiles, higher catalytic activities and altered specificities. Insight into water as a dielectric, the role of ions in electrostatic shielding and the field effects on...
Topics
- Buffers
- Catalysis
- Electricity
- Hydrogen-Ion Concentration
- Ions
- Kinetics
- Mutation
- Osmolar Concentration
- Structure-Activity Relationship
- Substrate Specificity
- Subtilisins
- Surface Properties