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Article

Variants of subtilisin BPN' with altered specificity profiles.

Biochemistry - 11 Jan 1994

Rheinnecker M, Eder J, Pandey P S, Fersht A R

Abstract excerpt

A strategy for increasing the size of the S4 binding pocket was used to improve the specificity of subtilisin BPN' toward substrates with large hydrophobic P4 side chains. This approach involves single and double amino acid replacements at positions 104, 107, and 126. Previously, alteration of I107 to glycine has been found to increase the specificity of subtilisin toward leucine, isoleucine, and phenylalanine as...

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PMID: 8286344
DOI: 10.1021/bi00167a029

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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