Variants of subtilisin BPN' with altered specificity profiles.

doi:10.1021/bi00167a029

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Article

Variants of subtilisin BPN' with altered specificity profiles.

Biochemistry - 11 Jan 1994

Rheinnecker M, Eder J, Pandey P S, Fersht A R

Abstract excerpt

A strategy for increasing the size of the S4 binding pocket was used to improve the specificity of subtilisin BPN' toward substrates with large hydrophobic P4 side chains. This approach involves single and double amino acid replacements at positions 104, 107, and 126. Previously, alteration of I1...

Topics

Amino Acid Sequence
Binding Sites
Genetic Variation
Kinetics
Molecular Sequence Data
Mutagenesis, Site-Directed
Point Mutation
Protein Conformation
Recombinant Proteins
Substrate Specificity
Subtilisins

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