Hydrolysis of small peptide substrates parallels binding of chymotrypsin inhibitor 2 for mutants of subtilisin BPN'.

doi:10.1016/0014-5793(93)80417-s

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Article

Hydrolysis of small peptide substrates parallels binding of chymotrypsin inhibitor 2 for mutants of subtilisin BPN'.

FEBS letters - 13 Dec 1993

Eder J, Rheinnecker M, Fersht A R

Abstract excerpt

Variants of subtilisin BPN' that possess improved specificity towards isoleucine compared with alanine at the P4 position of small peptide substrates, were analysed for their ability to bind chymotrypsin inhibitor 2. The binding of the inhibitor with isoleucine (wild-type) and with alanine as the...

Topics

Amino Acid Sequence
Base Sequence
Chymotrypsin
DNA
Hydrolysis
Molecular Sequence Data
Molecular Structure
Mutation
Peptides
Plant Proteins
Substrate Specificity
Subtilisins
Thermodynamics

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