Active-site titration of serine proteases using a fluoride ion selective electrode and sulfonyl fluoride inhibitors.

Abstract excerpt

We report a general procedure for the determination of active enzyme concentrations for serine proteases. The method relies on the measurement of fluoride ion released from sulfonyl fluorides upon reaction with the active-site serine using an ion selective electrode. The results have been independently confirmed by amino acid analyses of subtilisins and by spectrofluorometric and spectrophotometric titrations....

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