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Article

Active-site titration of serine proteases using a fluoride ion selective electrode and sulfonyl fluoride inhibitors.

Analytical biochemistry - 15 Nov 1996

Hsia C Y, Ganshaw G, Paech C, Murray C J

Abstract excerpt

We report a general procedure for the determination of active enzyme concentrations for serine proteases. The method relies on the measurement of fluoride ion released from sulfonyl fluorides upon reaction with the active-site serine using an ion selective electrode. The results have been indepen...

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Source

Identifiers

PMID: 8937565
DOI: 10.1006/abio.1996.0456

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 2 Sept 2026. Prepared record publication-8937565-2026-09-02.2. Manifest publication-graph-cohort-2026-09-02.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 2 Sept 2026.

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