Article
Retrovirus-mediated insertion of expressed and non-expressed genes at identical chromosomal locations.
Nucleic acids research - 25 May 1993
Berwin B, Barklis E
Abstract excerpt
During retrovirus replication, a cellularly derived tRNA is annealed to the viral RNA at the primer binding site (PBS) to prime reverse transcription, and both the tRNA and the PBS become copied and matched together on complementary proviral DNA strands prior to integration. Using a viral PBS sin...
Topics
- Base Sequence
- Blotting, Southern
- Cell Line
- Flow Cytometry
- Genes, Viral
- Histocytochemistry
- Methylation
- Molecular Sequence Data
- Moloney murine leukemia virus
- Mutation
- Nucleic Acid Hybridization
- Polymerase Chain Reaction
- RNA, Viral
- Recombinant Fusion Proteins
- Restriction Mapping
- Transfection
- beta-Galactosidase