Article
Retroviruses as genetic tools to isolate transcriptionally active chromosomal regions.
Environmental health perspectives - 1 Aug 1990
von Melchner H, Ruley H E
Abstract excerpt
By exploiting the ability of retroviruses to move genes into random sites of mammalian genomes and by exploiting some features of their replication, retrovirus vectors have been developed that select for instances in which the virus integrates into expressed genes. Since integrated proviruses tag transcriptionally active sites, the vectors provide a means to identify and isolate promoters active in different cell...
Topics
- Chromosomes
- Gene Expression Regulation, Viral
- Genetic Vectors
- Mutation
- Promoter Regions, Genetic
- Retroviridae
- Transcription, Genetic
- Virus Replication