Selective impairment of the synthesis of basic fibroblast growth factor binding domains of heparan sulphate in a COS cell mutant defective in N-sulphotransferase.

Abstract excerpt

N-Sulphation is a key step in the overall sulphation of heparan sulphate. We have isolated a COS cell-derived mutant, CM-15, that is impaired in its ability to bind to basic fibroblast growth factor (bFGF) and has a 2- to 3-fold reduction in N-sulphotransferase activity [Ishihara et al., (1992a) Anal. Biochem., 206, 400-407]. We now provide structural evidence that CM-15 is selectively impaired in the synthesis...

Topics

• Binding Sites
• Carbohydrate Sequence
• Cell Line
• Chromatography, Affinity
• Fibroblast Growth Factor 2
• Heparitin Sulfate
• Humans
• Molecular Sequence Data
• Mutation
• Peptide Fragments
• Sulfates