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Article

Heparan N-sulfatase: in vitro mutagenesis of potential N-glycosylation sites.

Biochemical and biophysical research communications - 9 Feb 2001

Di Natale P, Vanacore B, Daniele A, Esposito S

Abstract excerpt

Heparan N-sulfatase cDNA contains five potential N-glycosylation sites at Asn positions 41, 142, 151, 264, and 413. We used site-directed mutagenesis, substituting the codon of asparagine for glutamine, to eliminate selected glycosylation sites and then performed expression studies in COS-7 cells to determine the influence on the catalytic activity, lysosomal targeting, and glycosylation-phosphorylation of the...

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Identifiers

PMID: 11162662
DOI: 10.1006/bbrc.2001.4265

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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