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Article

Selection of COS cell mutants defective in the biosynthesis of heparan sulfate proteoglycan.

Analytical biochemistry - 1 Nov 1992

Ishihara M, Kiefer M C, Barr P J, Guo Y, Swiedler S J

Abstract excerpt

A simple procedure using human basic fibroblast growth factor (FGF) was utilized for the selection of COS cell mutants with defects in the biosynthesis or expression of heparan sulfate proteoglycan (HSPG). Our approach was based on the strong binding affinity exhibited by COS cells to human basic FGF that had been adsorbed to plastic dishes. Cell binding to basic FGF could be inhibited by heparin and heparan...

Topics

Animals
Cell Line
Fibroblast Growth Factor 2
Glycosaminoglycans
Heparan Sulfate Proteoglycans
Heparitin Sulfate
Kinetics
Mutation
Proteoglycans
Radioisotope Dilution Technique
Receptors, Fibroblast Growth Factor

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Sulfates

Sulfotransferases

Sulfur Radioisotopes

Source

Identifiers

PMID: 1443612
DOI: 10.1016/0003-2697(92)90385-k

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1443612-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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