Article
Assessment of the viral RNA sequence heterogeneity for control of OPV neurovirulence.
Developments in biological standardization - 1 Jan 1993
Chumakov K, Norwood L, Parker M, Dragunsky E, Taffs R, Ran Y, Ridge J, Levenbook I
Abstract excerpt
By using sensitive mutant analysis by PCR and restriction enzyme cleavage we have found that among several positions that differ between the wild-type and attenuated type 3 poliovirus genomes, only two positions, 472 and 2493, showed variability in vaccine lots. Of these two, only position 472 co...
Topics
- Animals
- Haplorhini
- Mutation
- Poliovirus
- Poliovirus Vaccine, Oral
- Predictive Value of Tests
- RNA, Viral
- Single-Blind Method
- Spinal Cord
- Virulence