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Article

A genetic selection elucidates structural determinants of arginine versus lysine specificity in trypsin.

Gene - 27 Dec 1993

Perona J J, Evnin L B, Craik C S

Abstract excerpt

A genetic selection has been used to isolate variants of the serine protease, trypsin (Tsn), altered in specificity toward lysine- and arginine-containing substrates. Growth of a lysine auxotroph of Escherichia coli was coupled to activation by Tsn of a non-nutritive source of lysine present in selective media. Nine Tsn variants possessing partial activities were isolated from a random library encompassing amino...

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Source

Identifiers

PMID: 8282194
DOI: 10.1016/0378-1119(93)90259-6

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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