Article
Alteration of the specificity of the Streptomyces subtilisin inhibitor by gene engineering.
Bio/technology (Nature Publishing Company) - 1 May 1990
Kojima S, Obata S, Kumagai I, Miura K
Abstract excerpt
We have altered the amino acid at the center of the reactive site (methionine 73) of Streptomyces subtilisin inhibitor (SSI) by site-directed and cassette mutagenesis. Replacement by lysine or arginine resulted in trypsin inhibitory activity, replacement only by lysine gave inhibition of lysyl endopeptidase, and replacement by tyrosine or tryptophan resulted in inhibition of alpha-chymotrypsin. The four mutant...
Topics
- Amino Acid Sequence
- Bacterial Proteins
- Base Sequence
- Genetic Engineering
- Methionine
- Molecular Sequence Data
- Mutation
- Serine Proteinase Inhibitors
- Streptomyces
- Substrate Specificity
- Subtilisins