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Article

Alteration of the specificity of the Streptomyces subtilisin inhibitor by gene engineering.

Bio/technology (Nature Publishing Company) - 1 May 1990

Kojima S, Obata S, Kumagai I, Miura K

Abstract excerpt

We have altered the amino acid at the center of the reactive site (methionine 73) of Streptomyces subtilisin inhibitor (SSI) by site-directed and cassette mutagenesis. Replacement by lysine or arginine resulted in trypsin inhibitory activity, replacement only by lysine gave inhibition of lysyl endopeptidase, and replacement by tyrosine or tryptophan resulted in inhibition of alpha-chymotrypsin. The four mutant...

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PMID: 1366538
DOI: 10.1038/nbt0590-449

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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