Article
Substrate specificity of trypsin investigated by using a genetic selection.
Proceedings of the National Academy of Sciences of the United States of America - 1 Sept 1990
Evnin L B, Vásquez J R, Craik C S
Abstract excerpt
The structural determinants of the primary substrate specificity of rat anionic trypsin were examined by using oligonucleotide-directed mutagenesis coupled to a genetic selection. A library was created that encoded trypsins substituted at amino acid positions 189 and 190 at the base of the substr...
Topics
- Amino Acid Sequence
- Animals
- Base Sequence
- Binding Sites
- Escherichia coli
- Gene Library
- Kinetics
- Molecular Sequence Data
- Mutation
- Oligonucleotide Probes
- Oligopeptides
- Plasmids
- Protein Conformation
- Rats
- Recombinant Proteins
- Substrate Specificity
- Trypsin