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Article

Substrate specificity of trypsin investigated by using a genetic selection.

Proceedings of the National Academy of Sciences of the United States of America - 1 Sept 1990

Evnin L B, Vásquez J R, Craik C S

Abstract excerpt

The structural determinants of the primary substrate specificity of rat anionic trypsin were examined by using oligonucleotide-directed mutagenesis coupled to a genetic selection. A library was created that encoded trypsins substituted at amino acid positions 189 and 190 at the base of the substrate binding pocket. A genetic selection, with a dynamic range of 5 orders of proteolytic activity, was used to search...

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Identifiers

PMID: 2204062
DOI: 10.1073/pnas.87.17.6659
PMCID: PMC54596

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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