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Article

Isolating substrates for an engineered alpha-lytic protease by phage display.

Journal of protein chemistry - 1 Feb 2003

Lien Samantha, Francis Geoffrey L, Graham Lloyd D, Wallace John C

Abstract excerpt

Panning of a substrate phage library with an alpha-lytic protease mutant showed that substrate phage display can be used to isolate sequences with improved protease sensitivity even for proteases of relatively broad specificity. Two panning experiments were performed with an engineered alpha-lytic protease mutant known to have a preference for cleavage after His or Met residues. Both experiments led to the...

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Identifiers

PMID: 12760420
DOI: 10.1023/a:1023475030579

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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