Article
Structural origins of substrate discrimination in trypsin and chymotrypsin.
Biochemistry - 7 Feb 1995
Perona J J, Hedstrom L, Rutter W J, Fletterick R J
Abstract excerpt
Converting the specificity of trypsin to that of chymotrypsin has been shown to require the exchange of amino acids in multiple portions of the protein, including two surface loops which do not directly contact the substrate. Crystallographic analysis of two mutant trypsins possessing chymotrypsi...
Topics
- Acylation
- Amino Acid Sequence
- Binding Sites
- Chymotrypsin
- Computer Simulation
- Crystallography
- Molecular Sequence Data
- Mutation
- Substrate Specificity
- Trypsin