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Article

Structural origins of substrate discrimination in trypsin and chymotrypsin.

Biochemistry - 7 Feb 1995

Perona J J, Hedstrom L, Rutter W J, Fletterick R J

Abstract excerpt

Converting the specificity of trypsin to that of chymotrypsin has been shown to require the exchange of amino acids in multiple portions of the protein, including two surface loops which do not directly contact the substrate. Crystallographic analysis of two mutant trypsins possessing chymotrypsin-like specificity now reveals that these distal surface loops alter function by directly determining the structure of...

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Source

Identifiers

PMID: 7849008
DOI: 10.1021/bi00005a004

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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