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Article

Engineering of protease variants exhibiting high catalytic activity and exquisite substrate selectivity.

Proceedings of the National Academy of Sciences of the United States of America - 10 May 2005

Varadarajan Navin, Gam Jongsik, Olsen Mark J, Georgiou George, Iverson Brent L

Abstract excerpt

The exquisite selectivity and catalytic activity of enzymes have been shaped by the effects of positive and negative selection pressure during the course of evolution. In contrast, enzyme variants engineered by using in vitro screening techniques to accept novel substrates typically display a higher degree of catalytic promiscuity and lower total turnover in comparison with their natural counterparts. Using...

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Identifiers

PMID: 15867160
DOI: 10.1073/pnas.0500063102
PMCID: PMC1100772

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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