Article
Purification and characterization of S1 mutants of alpha-lytic protease having altered catalytic properties.
Archives of biochemistry and biophysics - 1 Oct 1994
Haggett K D, Graham L D, Milner S J, Whittaker R G
Abstract excerpt
A procedure is described for purifying alpha-lytic protease and its mutants from culture supernatants of recombinant Escherichia coli. The method affords substantial amounts (approx. 80 mg) of homogeneous enzyme. We compared the cleavage preferences of wild-type alpha-lytic protease and of mutant...
Topics
- Amides
- Amino Acid Sequence
- Catalysis
- Enzyme Stability
- Escherichia coli
- Esters
- Hydrogen-Ion Concentration
- Hydrolysis
- Kinetics
- Molecular Sequence Data
- Mutation
- Recombinant Proteins
- Serine Endopeptidases
- Structure-Activity Relationship
- Substrate Specificity