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Article

Purification and characterization of S1 mutants of alpha-lytic protease having altered catalytic properties.

Archives of biochemistry and biophysics - 1 Oct 1994

Haggett K D, Graham L D, Milner S J, Whittaker R G

Abstract excerpt

A procedure is described for purifying alpha-lytic protease and its mutants from culture supernatants of recombinant Escherichia coli. The method affords substantial amounts (approx. 80 mg) of homogeneous enzyme. We compared the cleavage preferences of wild-type alpha-lytic protease and of mutants containing the substitutions Ala190 ("parent"), Ala190/Val192/His213/Met218 (mutant 1), Ala190/His213/Leu218 (mutant...

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Identifiers

PMID: 7944385
DOI: 10.1006/abbi.1994.1421

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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