Article
Structural basis for broad specificity in alpha-lytic protease mutants.
Biochemistry - 29 Oct 1991
Bone R, Fujishige A, Kettner C A, Agard D A
Abstract excerpt
Binding pocket mutants of alpha-lytic protease (Met 192----Ala and Met 213----Ala) have been constructed recently in an effort to create a protease specific for Met just prior to the scissile bond. Instead, mutation resulted in proteases with extraordinarily broad specificity profiles and high ac...
Topics
- Alanine
- Electricity
- Endopeptidases
- Escherichia coli
- Kinetics
- Methionine
- Models, Molecular
- Mutation
- Substrate Specificity
- X-Ray Diffraction