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Article

Structural basis for broad specificity in alpha-lytic protease mutants.

Biochemistry - 29 Oct 1991

Bone R, Fujishige A, Kettner C A, Agard D A

Abstract excerpt

Binding pocket mutants of alpha-lytic protease (Met 192----Ala and Met 213----Ala) have been constructed recently in an effort to create a protease specific for Met just prior to the scissile bond. Instead, mutation resulted in proteases with extraordinarily broad specificity profiles and high activity [Bone, R., Silen, J. L., & Agard, D. A. (1989) Nature 339, 191-195]. To understand the structural basis for the...

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PMID: 1931963
DOI: 10.1021/bi00107a005

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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