Article
N-glycosylation of human acetylcholinesterase: effects on activity, stability and biosynthesis.
The Biochemical journal - 15 Dec 1993
Velan B, Kronman C, Ordentlich A, Flashner Y, Leitner M, Cohen S, Shafferman A
Abstract excerpt
The role of N-glycosylation in the function of human acetylcholinesterase (HuAChE) was examined by site-directed mutagenesis (Asn to Gln substitution) of the three potential N-glycosylation sites Asn-265, Asn-350 and Asn-464. Analysis of HuAChE mutants, defective in a single or multiple N-glycosy...
Topics
- Acetylcholinesterase
- Amino Acid Sequence
- Catalysis
- Cells, Cultured
- Cholinesterase Inhibitors
- Edrophonium
- Enzyme Stability
- Glycosylation
- Humans
- Molecular Sequence Data
- Mutation
- Oligosaccharides
- Quaternary Ammonium Compounds
- Recombinant Proteins
- Transfection