Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Mutations in the conserved loop of human U5 snRNA generate use of novel cryptic 5' splice sites in vivo.

The EMBO journal - 15 Dec 1993

Cortes J J, Sontheimer E J, Seiwert S D, Steitz J A

Abstract excerpt

We have analyzed base pairing interactions between the U5 snRNA and 5' exon sequences during pre-mRNA splicing in a mammalian in vivo system. We constructed synthetic U5 genes with mutations that alter four bases (C3, U4, U5 and U6) within the invariant 9 nt U5 sequence GCCUUUUAC; transient transfection of HeLa cells with these U5 sequences cloned into a U1 expression vector yielded high levels of the mutant...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Base Sequence
Share to Globins
Share to Molecular Sequence Data

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Mutation

Share to Oligodeoxyribonucleotides

Share to Rabbits

Share to RNA, Messenger

Share to RNA Splicing

Source

Open in PubMed Open full text

Identifiers

PMID: 8262061
DOI: 10.1002/j.1460-2075.1993.tb06213.x
PMCID: PMC413781

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Mutations in the conserved loop of human U5 snRNA generate use of novel cryptic 5' splice sites in vivo.PMID 8262061Read ↗

U5 snRNA interactions with exons ensure splicing precisionSemantic proximity Read ↗

Mechanism for cryptic splice site activation during pre-mRNA splicing.Semantic proximity Read ↗

In vitro reconstitution of yeast splicing with U4 snRNA reveals multiple roles for the 3' stem-loop.Semantic proximity Read ↗

How to Design U1 snRNA Molecules for Splicing Rescue.Semantic proximity Read ↗

Restoration of correct splicing of thalassemic beta-globin pre-mRNA by modified U1 snRNAs.Semantic proximity Read ↗

The phylogenetically invariant ACAGAGA and AGC sequences of U6 small nuclear RNA are more tolerant of mutation in human cells than in Saccharomyces cerevisiae.Semantic proximity Read ↗

Mutations in yeast U5 snRNA alter the specificity of 5' splice-site cleavage.Semantic proximity Read ↗

An intronic splicing enhancer binds U1 snRNPs to enhance splicing and select 5' splice sites.Semantic proximity Read ↗

Functionally redundant interactions between U2 and U6 spliceosomal snRNAs.Semantic proximity Read ↗

A U6 snRNA gene with an internal promoter is juxtaposed to an snRNP protein sequence within an intron of a human G protein gene.Semantic proximity Read ↗

Mutation in the U2 snRNA influences exon interactions of U5 snRNA loop 1 during pre-mRNA splicing.Semantic proximity Read ↗

Invariant U2 RNA sequences bordering the branchpoint recognition region are essential for interaction with yeast SF3a and SF3b subunits.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.