Mutations in the conserved loop of human U5 snRNA generate use of novel cryptic 5' splice sites in vivo.

doi:10.1002/j.1460-2075.1993.tb06213.x

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Article

Mutations in the conserved loop of human U5 snRNA generate use of novel cryptic 5' splice sites in vivo.

The EMBO journal - 15 Dec 1993

Cortes J J, Sontheimer E J, Seiwert S D, Steitz J A

Abstract excerpt

We have analyzed base pairing interactions between the U5 snRNA and 5' exon sequences during pre-mRNA splicing in a mammalian in vivo system. We constructed synthetic U5 genes with mutations that alter four bases (C3, U4, U5 and U6) within the invariant 9 nt U5 sequence GCCUUUUAC; transient trans...

Topics

Animals
Base Sequence
Globins
Humans
Molecular Sequence Data
Mutation
Nucleic Acid Precursors
Oligodeoxyribonucleotides
RNA Splicing
RNA, Messenger
RNA, Small Nuclear
Rabbits

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