An intronic splicing enhancer binds U1 snRNPs to enhance splicing and select 5' splice sites.

doi:10.1128/MCB.20.24.9225-9235.2000

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Article

An intronic splicing enhancer binds U1 snRNPs to enhance splicing and select 5' splice sites.

Molecular and cellular biology - 1 Dec 2000

McCullough A J, Berget S M

Abstract excerpt

Intronic G triplets are frequently located adjacent to 5' splice sites in vertebrate pre-mRNAs and have been correlated with splicing efficiency and specificity via a mechanism that activates upstream 5' splice sites in exons containing duplicated sites (26). Using an intron dependent upon G triplets for maximal activity and 5' splice site specificity, we determined that these elements bind U1 snRNPs via base...

Topics

Base Pairing
Base Sequence
Cross-Linking Reagents
Electrophoresis
Enhancer Elements, Genetic
Ficusin
Globins
HeLa Cells
Humans
Introns
Molecular Sequence Data
Mutation
Nuclear Proteins
Nucleic Acid Conformation
Phosphoproteins
RNA
RNA Splice Sites
RNA Splicing

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Identifiers

PMID: 11094074
DOI: 10.1128/MCB.20.24.9225-9235.2000
PMCID: PMC102180

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