Article
Exploiting structural differences among heteroduplex molecules to simplify genotyping the DQA1 and DQB1 alleles in human lymphocyte typing.
Nucleic acids research - 25 Sept 1993
Zimmerman P A, Carrington M N, Nutman T B
Abstract excerpt
A novel approach to DNA probe hybridization and heteroduplex analysis, termed directed heteroduplex analysis (DHDA) is presented here to illustrate its utility in simplification of human lymphocyte antigen (HLA)-typing. By strategic labeling of single-stranded probe sequences, DHDA allows the ide...
Topics
- Alleles
- Base Sequence
- DNA Primers
- Electrophoresis, Polyacrylamide Gel
- HLA-DQ Antigens
- HLA-DQ alpha-Chains
- HLA-DQ beta-Chains
- Histocompatibility Testing
- Humans
- Molecular Sequence Data
- Nucleic Acid Hybridization
- Polymerase Chain Reaction
- Polymorphism, Genetic