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Article

Rapid HLA-DPB typing using enzymatically amplified DNA and nonradioactive sequence-specific oligonucleotide probes.

Immunogenetics - 1 Jan 1990

Bugawan T L, Begovich A B, Erlich H A

Abstract excerpt

A simple and rapid method for characterizing the polymorphism at the HLA-DPB1 locus has been developed. The procedure involves the selective amplification of the polymorphic second exon of the DPB1 locus by the polymerase chain reaction (PCR), followed by hybridization of the amplified DNA with 15 nonisotopic sequence-specific oligonucleotide probes. There are no sequences within the second exon of the DPB1 locus...

Topics

Alleles
Amino Acid Sequence
Base Sequence
Chromosomes, Human, Pair 6
Female
HLA-DP Antigens
Haplotypes
Humans
Immunoblotting
Male
Molecular Sequence Data
Oligonucleotide Probes

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Polymerase Chain Reaction

Polymorphism, Genetic

Sequence Homology, Nucleic Acid

Source

Identifiers

PMID: 2242906
DOI: 10.1007/BF00187094

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2242906-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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