Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

A rapid HLA-DRB1*04 subtyping method using PCR and DNA heteroduplex generators.

Tissue antigens - 1 Apr 1996

Savage D A, Tang J P, Wood N A, Evans J, Bidwell J L, Wee J L, Oei A A, Hui K M

Abstract excerpt

We describe here a rapid polymerase chain reaction (PCR)-based method for the identification of HLA-DRB1*0401-*0412 alleles. The method is based on the generation of specific DNA heteroduplex patterns between PCR products derived from selective group-specific amplification of the various DRB1*04 alleles and PCR products derived from two synthetic DNA heteroduplex generator (DHG) molecules following non-denaturing...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Alleles
Share to Base Sequence
Share to Cell Line

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to HLA-DR Antigens

Share to HLA-DRB1 Chains

Share to Molecular Sequence Data

Share to Polymerase Chain Reaction

Source

Identifiers

PMID: 8773317
DOI: 10.1111/j.1399-0039.1996.tb02555.x

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

A rapid HLA-DRB1*04 subtyping method using PCR and DNA heteroduplex generators.PMID 8773317Read ↗

HLA-DRB1*01 subtyping by heteroduplex analysis.Semantic proximity Read ↗

HLA-DQA1 and -DQB1 genotyping by PCR-RFLP, heteroduplex and homoduplex analysis.Semantic proximity Read ↗

Detection of beta-thalassaemia mutations using DNA heteroduplex generator molecules.Semantic proximity Read ↗

A simple and economical DRB1 typing procedure combining group-specific amplification, DNA heteroduplex and enzyme restriction analysis.Semantic proximity Read ↗

Identification of the HLA-DRB1*04, -DRB1*07, and -DRB1*09 alleles by PCR amplification with sequence-specific primers (PCR-SSP) in 2 hours.Semantic proximity Read ↗

Subgrouping of DR4 alleles by DNA heteroduplex analysis.Semantic proximity Read ↗

Rapid typing of HLA-DQB1 DNA polymorphism using nonradioactive oligonucleotide probes and amplified DNA.Semantic proximity Read ↗

High-resolution HLA-DRB typing using denaturing gradient gel electrophoresis and direct sequencing.Semantic proximity Read ↗

Exploiting structural differences among heteroduplex molecules to simplify genotyping the DQA1 and DQB1 alleles in human lymphocyte typing.Semantic proximity Read ↗

A practical approach to HLA-DR genomic typing by heteroduplex analysis and a selective cleavage at position 86.Semantic proximity Read ↗

Discrimination of human HLA-DRB1 alleles by PCR-SSCP (single-strand conformation polymorphism) method.Semantic proximity Read ↗

HLA-DR typing using DNA amplification by the polymerase chain reaction and sequential hybridization to sequence-specific oligonucleotide probes.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.