A rapid HLA-DRB1*04 subtyping method using PCR and DNA heteroduplex generators.

doi:10.1111/j.1399-0039.1996.tb02555.x

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Article

A rapid HLA-DRB1*04 subtyping method using PCR and DNA heteroduplex generators.

Tissue antigens - 1 Apr 1996

Savage D A, Tang J P, Wood N A, Evans J, Bidwell J L, Wee J L, Oei A A, Hui K M

Abstract excerpt

We describe here a rapid polymerase chain reaction (PCR)-based method for the identification of HLA-DRB1*0401-*0412 alleles. The method is based on the generation of specific DNA heteroduplex patterns between PCR products derived from selective group-specific amplification of the various DRB1*04...

Topics

Alleles
Base Sequence
Cell Line
DNA
HLA-DR Antigens
HLA-DRB1 Chains
Humans
Molecular Sequence Data
Nucleic Acid Heteroduplexes
Polymerase Chain Reaction

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