Article
A practical approach to HLA-DR genomic typing by heteroduplex analysis and a selective cleavage at position 86.
Human immunology - 1 May 1994
el-Borai M H, D'Alfonso S, Mazzola G, Fasano M E
Abstract excerpt
A common problem facing HLA-typing laboratories is to substitute genomic typing for serology without having to handle a large number of oligoprobes, primers, or restriction enzymes. A protocol is described for HLA-DRB1 genomic typing using a combination of PCR amplification, DNA heteroduplex anal...
Topics
- Alleles
- Base Sequence
- Blood Cells
- DNA
- Genes, MHC Class II
- HLA-DR Antigens
- Histocompatibility Testing
- Humans
- Molecular Sequence Data
- Nucleic Acid Heteroduplexes
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length