Article
A practical approach to HLA-DR genomic typing by heteroduplex analysis and a selective cleavage at position 86.
Human immunology - 1 May 1994
el-Borai M H, D'Alfonso S, Mazzola G, Fasano M E
Abstract excerpt
A common problem facing HLA-typing laboratories is to substitute genomic typing for serology without having to handle a large number of oligoprobes, primers, or restriction enzymes. A protocol is described for HLA-DRB1 genomic typing using a combination of PCR amplification, DNA heteroduplex analysis, and restriction enzymes. Because the core of the procedure is the analysis of the DNA heteroduplexs, it shall be...
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