Article
A combination of two distinct in vitro amplification procedures for DNA typing of HLA-DRB and -DQB 1 alleles.
Vox sanguinis - 1 Jan 1995
Fischer G F, Faé I, Petrasek M, Moser S
Abstract excerpt
The differential hybridisation of oligonucleotide probes to polymerase chain reaction (PCR)-amplified DNA has become a standard procedure for tissue typing. We describe a typing method in which differential ligation replaces differential hybridisation, which is a significant simplification of this strategy. After amplification by the PCR two labelled, sequence-specific oligonucleotides hybridise, in the fluid...
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