Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

A simple and economical DRB1 typing procedure combining group-specific amplification, DNA heteroduplex and enzyme restriction analysis.

Tissue antigens - 1 May 1994

D'Amato M, Sorrentino R

Abstract excerpt

A new procedure for HLA-DRB1 typing is proposed. The method combines six group-specific amplifications with heteroduplex analysis and, in some cases, enzyme restriction analysis. This technique, which is as discriminative as oligotyping, is simple, economical and does not require probes. These characteristics make this approach a valid alternative to other HLA genomic typing procedures, especially in those cases...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Alleles
Share to Base Sequence
Share to Cell Line

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Gene Amplification

Share to Histocompatibility Antigens Class II

Share to HLA-DR Antigens

Share to Molecular Sequence Data

Source

Identifiers

PMID: 7940498
DOI: 10.1111/j.1399-0039.1994.tb02343.x

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

A simple and economical DRB1 typing procedure combining group-specific amplification, DNA heteroduplex and enzyme restriction analysis.PMID 7940498Read ↗

A new approach to HLA-DPB1 typing combining DNA heteroduplex analysis with allele-specific amplification and enzyme restriction.Semantic proximity Read ↗

An HLA-DR typing protocol using group-specific PCR-amplification followed by restriction enzyme digests.Semantic proximity Read ↗

High resolution genetic typing of the class II HLA-DRB1 locus using group-specific amplification and SSO-hybridisation in microplates.Semantic proximity Read ↗

An improved strategy for HLA-DRB1 subtyping by digestion of PCR-amplified DNA with allele-specific restriction endonucleases.Semantic proximity Read ↗

A rapid HLA-DRB1*04 subtyping method using PCR and DNA heteroduplex generators.Semantic proximity Read ↗

A practical approach to HLA-DR genomic typing by heteroduplex analysis and a selective cleavage at position 86.Semantic proximity Read ↗

Determination of DRB alleles using PCR amplification and allele-specific primers.Semantic proximity Read ↗

Group-specific amplification of HLA-DQA1 revealed a number of genomic full-length sequences including the novel HLA alleles DQA1*01:10 and DQA1*01:11.Semantic proximity Read ↗

New high resolution typing strategy for HLA-A locus alleles based on dye terminator sequencing of haplotypic group-specific PCR-amplicons of exon 2 and exon 3.Semantic proximity Read ↗

HLA-DRB1*01 subtyping by heteroduplex analysis.Semantic proximity Read ↗

Determination of DQB1 alleles using PCR amplification and allele-specific primers.Semantic proximity Read ↗

Complete HLA-A DNA typing using the PCR-RFLP method combined with allele group- and sequence-specific amplification.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.