Article
A simple and economical DRB1 typing procedure combining group-specific amplification, DNA heteroduplex and enzyme restriction analysis.
Tissue antigens - 1 May 1994
D'Amato M, Sorrentino R
Abstract excerpt
A new procedure for HLA-DRB1 typing is proposed. The method combines six group-specific amplifications with heteroduplex analysis and, in some cases, enzyme restriction analysis. This technique, which is as discriminative as oligotyping, is simple, economical and does not require probes. These ch...
Topics
- Alleles
- Base Sequence
- Cell Line
- DNA
- DNA Restriction-Modification Enzymes
- Gene Amplification
- HLA-DR Antigens
- HLA-DRB1 Chains
- HLA-DRB3 Chains
- Histocompatibility Antigens Class II
- Histocompatibility Testing
- Humans
- Molecular Sequence Data
- Nucleic Acid Heteroduplexes