Article
HLA-DRB1*01 subtyping by heteroduplex analysis.
Tissue antigens - 1 Feb 1995
Savage D A, Wood N A, Bidwell J L, Hui K M
Abstract excerpt
In this report we describe an alternative method for the identification of the four known HLA-DRB1*01 alleles, which is based on the generation of unique heteroduplex patterns between the different DRB*01 alleles and a synthetic DNA heteroduplex generator (DHG) molecule. The method is technically...
Topics
- Alleles
- Base Sequence
- Electrophoresis, Polyacrylamide Gel
- Genes, MHC Class II
- HLA-DR Antigens
- HLA-DRB1 Chains
- Humans
- Molecular Sequence Data
- Nucleic Acid Heteroduplexes
- Polymerase Chain Reaction
- Sequence Alignment
- Sequence Homology, Nucleic Acid