Article
Genetic analysis of yeast strains lacking negative feedback control: a one-step method for positive selection and cloning of carbamoylphosphate synthetase-aspartate transcarbamoylase mutants unable to respond to UTP.
Molecular & general genetics : MGG - 1 Oct 1993
Jaquet L, Lollier M, Souciet J L, Potier S
Abstract excerpt
We have undertaken an in vivo genetic approach to the analysis of negative feedback control by uridine triphosphate (UTP) of the yeast carbamoylphosphate synthetase-aspartate transcarbamoylase multifunctional protein (CPSase-ATCase). Using an analog of uracil, 5-fluorouracil, we have constructed...
Topics
- Allosteric Regulation
- Amino Acids
- Aspartate Carbamoyltransferase
- Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing)
- Cloning, Molecular
- DNA Mutational Analysis
- Enzyme Stability
- Feedback
- Fluorouracil
- Genes, Dominant
- Hot Temperature
- Multienzyme Complexes
- Mutation
- Saccharomyces cerevisiae