Article
Reactivation of the ATCase domain of the URA2 gene complex: a positive selection method for Ty insertions and chromosomal rearrangements in Saccharomyces cerevisiae.
Molecular & general genetics : MGG - 20 Mar 1995
Roelants F, Potier S, Souciet J L, de Montigny J
Abstract excerpt
Genetic rearrangements such as deletions or duplications of DNA sequences are rarely detected in the yeast Saccharomyces cerevisiae. We have developed a screening system using the URA2 gene coding for the bi-functional CPSase-ATCase (carbamyl phosphate synthetase - aspartate transcarbamylase) to...
Topics
- Aspartate Carbamoyltransferase
- Base Sequence
- Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing)
- Chromosome Deletion
- DNA Transposable Elements
- Gene Rearrangement
- Genes, Fungal
- Molecular Sequence Data
- Multienzyme Complexes
- Mutation
- Polymerase Chain Reaction
- Restriction Mapping
- Saccharomyces cerevisiae