Article
A new mutation delivery system for genome-scale approaches in Bacillus subtilis.
Molecular microbiology - 1 Oct 2002
Fabret Céline, Ehrlich S Dusko, Noirot Philippe
Abstract excerpt
In Bacillus subtilis, although many genetic tools have been developed, gene replacement remains labour-intensive and not compatible with large-scale approaches. We have developed a new one-step gene replacement procedure that allows rapid alteration of any gene sequence or multiple gene sequences in B. subtilis without altering the chromosome in any other way. This novel approach relies on the use of upp, which...
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