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Article

Evolutionary optimization of the catalytic properties of a DNA-cleaving ribozyme.

Biochemistry - 17 May 1994

Tsang J, Joyce G F

Abstract excerpt

In a previous study [Beaudry, A. A., & Joyce, G. F. (1992) Science 257, 635-641], an in vitro evolution procedure was used to obtain variants of the Tetrahymena ribozyme with 100-fold improved ability to cleave a target single-stranded DNA under physiologic conditions. Here we report continuation of the in vitro evolution process to achieve 10(5)-fold overall improvement in DNA-cleavage activity. In addition, we...

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Identifiers

PMID: 8180226
DOI: 10.1021/bi00185a038

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