Evolutionary optimization of the catalytic properties of a DNA-cleaving ribozyme.

doi:10.1021/bi00185a038

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Article

Evolutionary optimization of the catalytic properties of a DNA-cleaving ribozyme.

Biochemistry - 17 May 1994

Tsang J, Joyce G F

Abstract excerpt

In a previous study [Beaudry, A. A., & Joyce, G. F. (1992) Science 257, 635-641], an in vitro evolution procedure was used to obtain variants of the Tetrahymena ribozyme with 100-fold improved ability to cleave a target single-stranded DNA under physiologic conditions. Here we report continuation...

Topics

Animals
Base Sequence
Binding Sites
Biological Evolution
Catalysis
DNA
Hydrolysis
Kinetics
Molecular Sequence Data
Mutation
Nucleic Acid Conformation
RNA, Catalytic
RNA, Protozoan
Tetrahymena thermophila

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