Article
Coupling of ATPase activity and motility in smooth muscle myosin is mediated by the regulatory light chain.
The Journal of cell biology - 1 Mar 1994
Trybus K M, Waller G S, Chatman T A
Abstract excerpt
Smooth muscle myosin acts as a molecular motor only if the regulatory light chain (RLC) is phosphorylated. This subunit can be removed from myosin by a novel method involving the use of trifluoperazine. The motility of RLC-deficient myosin is very slow, but native properties are restored when RLC is rebound. Truncating 6 residues from the COOH terminus of the RLC had no effect on phosphorylated myosin's motor...
Topics
- Actins
- Adenosine Triphosphate
- Amino Acid Sequence
- Molecular Sequence Data
- Muscle, Smooth
- Mutation
- Myosins
- Phosphorylation