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Article

Site-directed spin labeling reveals a conformational switch in the phosphorylation domain of smooth muscle myosin.

Proceedings of the National Academy of Sciences of the United States of America - 15 Mar 2005

Nelson Wendy D, Blakely Sarah E, Nesmelov Yuri E, Thomas David D

Abstract excerpt

We have used site-directed spin labeling and EPR spectroscopy to detect structural changes within the regulatory light chain (RLC) of smooth muscle myosin upon phosphorylation. Smooth muscle contraction is activated by phosphorylation of S19 on RLC, but the structural basis of this process is unknown. There is no crystal structure containing a phosphorylated RLC, and there is no crystal structure for the...

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Identifiers

PMID: 15753305
DOI: 10.1073/pnas.0401664102
PMCID: PMC554790

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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