Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

A dominant negative mutation in the conserved RNA helicase motif 'SAT' causes splicing factor PRP2 to stall in spliceosomes.

The EMBO journal - 15 Feb 1994

Plumpton M, McGarvey M, Beggs J D

Abstract excerpt

To characterize sequences in the RNA helicase-like PRP2 protein of Saccharomyces cerevisiae that are essential for its function in pre-mRNA splicing, a pool of random PRP2 mutants was generated. A dominant negative allele was isolated which, when overexpressed in a wild-type yeast strain, inhibited cell growth by causing a defect in pre-mRNA splicing. This defect was partially alleviated by simultaneous...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Base Sequence
Share to Electrophoresis, Polyacrylamide Gel
Share to Fungal Proteins

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Genes, Dominant

Share to Molecular Sequence Data

Share to Mutation

Share to Saccharomyces cerevisiae

Share to Saccharomyces cerevisiae Proteins

Source

Open in PubMed Open full text

Identifiers

PMID: 8112301
DOI: 10.1002/j.1460-2075.1994.tb06331.x
PMCID: PMC394887

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

A dominant negative mutation in the conserved RNA helicase motif 'SAT' causes splicing factor PRP2 to stall in spliceosomes.PMID 8112301Read ↗

Dominant negative mutants of the yeast splicing factor Prp2 map to a putative cleft region in the helicase domain of DExD/H-box proteins.Semantic proximity Read ↗

Interaction between a G-patch protein and a spliceosomal DEXD/H-box ATPase that is critical for splicing.Semantic proximity Read ↗

A cold-sensitive mRNA splicing mutant is a member of the RNA helicase gene family.Semantic proximity Read ↗

Motifs IV and V in the DEAH box splicing factor Prp22 are important for RNA unwinding, and helicase-defective Prp22 mutants are suppressed by Prp8.Semantic proximity Read ↗

Inhibition of a spliceosome turnover pathway suppresses splicing defects.Semantic proximity Read ↗

A suppressor of a yeast splicing mutation (prp8-1) encodes a putative ATP-dependent RNA helicase.Semantic proximity Read ↗

The first ATPase domain of the yeast 246-kDa protein is required for in vivo unwinding of the U4/U6 duplex.Semantic proximity Read ↗

RNA helicase dynamics in pre-mRNA splicing.Semantic proximity Read ↗

Genome-wide analysis of pre-mRNA splicing: intron features govern the requirement for the second-step factor, Prp17 in Saccharomyces cerevisiae and Schizosaccharomyces pombe.Semantic proximity Read ↗

Extragenic suppressors of Saccharomyces cerevisiae prp4 mutations identify a negative regulator of PRP genes.Semantic proximity Read ↗

How Slu7 and Prp18 cooperate in the second step of yeast pre-mRNA splicing.Semantic proximity Read ↗

Prp45 affects Prp22 partition in spliceosomal complexes and splicing efficiency of non-consensus substrates.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.