Article
A dominant negative mutation in the conserved RNA helicase motif 'SAT' causes splicing factor PRP2 to stall in spliceosomes.
The EMBO journal - 15 Feb 1994
Plumpton M, McGarvey M, Beggs J D
Abstract excerpt
To characterize sequences in the RNA helicase-like PRP2 protein of Saccharomyces cerevisiae that are essential for its function in pre-mRNA splicing, a pool of random PRP2 mutants was generated. A dominant negative allele was isolated which, when overexpressed in a wild-type yeast strain, inhibited cell growth by causing a defect in pre-mRNA splicing. This defect was partially alleviated by simultaneous...
Topics
- Base Sequence
- Chromatography, Affinity
- Conserved Sequence
- DEAD-box RNA Helicases
- Electrophoresis, Polyacrylamide Gel
- Fungal Proteins
- Genes, Dominant
- Leucine
- Molecular Sequence Data
- Mutation
- Oligodeoxyribonucleotides