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Article

Dominant negative mutants of the yeast splicing factor Prp2 map to a putative cleft region in the helicase domain of DExD/H-box proteins.

RNA (New York, N.Y.) - 1 Aug 2000

Edwalds-Gilbert G, Kim D H, Kim S H, Tseng Y H, Yu Y, Lin R J

Abstract excerpt

The Prp2 protein of Saccharomyces cerevisiae is an RNA-dependent ATPase required before the first transesterification reaction in pre-mRNA splicing. Prp2 binds to the spliceosome in the absence of ATP and is released following ATP hydrolysis. We determined what regions in Prp2 are essential for release from the spliceosome by analyzing dominant negative mutants in vivo and in vitro. We made mutations in conserved...

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Identifiers

PMID: 10943890
DOI: 10.1017/s1355838200992483
PMCID: PMC1369985

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

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Research trail→

Click a neighbor to travelStep 1 · 12 closest

Dominant negative mutants of the yeast splicing factor Prp2 map to a putative cleft region in the helicase domain of DExD/H-box proteins.PMID 10943890Read ↗

Interaction between a G-patch protein and a spliceosomal DEXD/H-box ATPase that is critical for splicing.Semantic proximity Read ↗

A dominant negative mutation in the conserved RNA helicase motif 'SAT' causes splicing factor PRP2 to stall in spliceosomes.Semantic proximity Read ↗

Motifs IV and V in the DEAH box splicing factor Prp22 are important for RNA unwinding, and helicase-defective Prp22 mutants are suppressed by Prp8.Semantic proximity Read ↗

The DExD/H-box ATPase Prp2p destabilizes and proofreads the catalytic RNA core of the spliceosome.Semantic proximity Read ↗

A cold-sensitive mRNA splicing mutant is a member of the RNA helicase gene family.Semantic proximity Read ↗

RNA helicase dynamics in pre-mRNA splicing.Semantic proximity Read ↗

The final stages of spliceosome maturation require Spp2p that can interact with the DEAH box protein Prp2p and promote step 1 of splicing.Semantic proximity Read ↗

Mutations in PRP43 that uncouple RNA-dependent NTPase activity and pre-mRNA splicing function.Semantic proximity Read ↗

The first ATPase domain of the yeast 246-kDa protein is required for in vivo unwinding of the U4/U6 duplex.Semantic proximity Read ↗

Inhibition of a spliceosome turnover pathway suppresses splicing defects.Semantic proximity Read ↗

A dominant negative mutation in a spliceosomal ATPase affects ATP hydrolysis but not binding to the spliceosome.Semantic proximity Read ↗

Prp43 is an essential RNA-dependent ATPase required for release of lariat-intron from the spliceosome.Semantic proximity Read ↗

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