Article
The first ATPase domain of the yeast 246-kDa protein is required for in vivo unwinding of the U4/U6 duplex.
RNA (New York, N.Y.) - 1 Jul 1999
Kim D H, Rossi J J
Abstract excerpt
The yeast PRP44 gene, alternatively named as BRR2, SLT22, RSS1, or SNU246, encodes a 246-kDa protein with putative RNA helicase function during pre-mRNA splicing. The protein is a typical DEAD/H family member, but unlike most other members of this family, it contains two putative RNA helicase domains, each with a highly conserved ATPase motif. Prior to this study little was known about functional roles for these...
Topics
- Adenosine Triphosphatases
- Base Sequence
- DNA Primers
- Fungal Proteins
- Genes, Dominant
- Mutagenesis, Site-Directed
- Mutation
- Phenotype
- RNA Helicases
- RNA Splicing
- RNA, Double-Stranded
- Repressor Proteins
- Ribonucleoprotein, U4-U6 Small Nuclear
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins