Article
Activation of the K-ras gene by insertion mutations in chemically induced rat renal mesenchymal tumors.
Oncogene - 1 Sept 1994
Higinbotham K G, Rice J M, Buzard G S, Perantoni A O
Abstract excerpt
Previously we reported the detection of transforming K-ras sequences in methyl(methoxymethyl)nitrosamine (DMN-OMe)-induced rat renal mesenchymal tumors by NIH3T3 transfection assays. Subsequent analysis by selective oligonucleotide hybridization revealed a variety of activating point mutations in codon 12 of K-ras in most of these tumors and in their NIH3T3 transformants, but in some, point mutations could not be...
Topics
- 3T3 Cells
- Animals
- Base Sequence
- Gene Expression Regulation, Neoplastic
- Genes, ras
- Kidney Neoplasms
- Mice
- Molecular Sequence Data
- Mutation
- Rats
- Rats, Inbred F344