Article
Deletion of a DNA polymerase beta gene segment in T cells using cell type-specific gene targeting.
Science (New York, N.Y.) - 1 Jul 1994
Gu H, Marth J D, Orban P C, Mossmann H, Rajewsky K
Abstract excerpt
Deletion of the promoter and the first exon of the DNA polymerase beta gene (pol beta) in the mouse germ line results in a lethal phenotype. With the use of the bacteriophage-derived, site-specific recombinase Cre in a transgenic approach, the same mutation can be selectively introduced into a particular cellular compartment-in this case, T cells. The impact of the mutation on those cells can then be analyzed...
Topics
- Animals
- DNA Nucleotidyltransferases
- DNA Polymerase I
- Female
- Gene Deletion
- Genetic Engineering
- Homozygote
- Integrases
- Male
- Mice
- Mice, Knockout
- Mice, Transgenic