Wide-ranging and unexpected consequences of altered Pol II catalytic activity in vivo.

Abstract excerpt

Here we employ a set of RNA Polymerase II (Pol II) activity mutants to determine the consequences of increased or decreased Pol II catalysis on gene expression in Saccharomyces cerevisiae. We find that alteration of Pol II catalytic rate, either fast or slow, leads to decreased Pol II occupancy and apparent reduction in elongation rate in vivo. However, we also find that determination of elongation rate in vivo...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Biocatalysis
• Share to Chromatin Immunoprecipitation
• Share to Gene Expression Regulation, Fungal