Article
A single mutation in bacteriophage T4 DNA polymerase (A737V, tsL141) decreases its processivity as a polymerase and increases its processivity as a 3'-->5' exonuclease.
The Journal of biological chemistry - 7 Jan 1994
Spacciapoli P, Nossal N G
Abstract excerpt
The bacteriophage T4 DNA polymerase mutant A737V (tsL141 and tsCB120) was originally characterized as temperature-sensitive for DNA replication and an antimutator for transition mutations. Its antimutator phenotype is suppressed by the L771F mutation (Reha-Krantz, L. J., Stocki, S., Nonay, R., an...
Topics
- Amino Acid Sequence
- Bacteriophage T4
- Base Sequence
- Cloning, Molecular
- DNA-Directed DNA Polymerase
- Exodeoxyribonuclease V
- Exodeoxyribonucleases
- Molecular Sequence Data
- Mutation
- Oligodeoxyribonucleotides
- Templates, Genetic