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Article

The allele-specific synthetic lethality of prlA-prlG double mutants predicts interactive domains of SecY and SecE.

The EMBO journal - 1 Mar 1995

Flower A M, Osborne R S, Silhavy T J

Abstract excerpt

The secretion of proteins from the cytoplasm of Escherichia coli requires the interaction of two integral inner membrane components, SecY and SecE. We have devised a genetic approach to probe the molecular nature of the SecY-SecE interaction. Suppressor alleles of secY and secE, termed prlA and prlG, respectively, were analyzed in pair-wise combinations for synthetic phenotypes. From a total of 115 combinations,...

Topics

Alleles
Amino Acid Sequence
Bacterial Proteins
Base Sequence
Cell Membrane
Escherichia coli Proteins
Genes, Bacterial
Genes, Lethal
Genes, Recessive
Genetic Complementation Test
Membrane Proteins

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Molecular Sequence Data

SEC Translocation Channels

Suppression, Genetic

Source

Open in PubMed Open full text

Identifiers

PMID: 7889938
DOI: 10.1002/j.1460-2075.1995.tb07070.x
PMCID: PMC398161

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-7889938-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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The allele-specific synthetic lethality of prlA-prlG double mutants predicts interactive domains of SecY and SecE.PMID 7889938Read ↗

PrlA suppressor mutations cluster in regions corresponding to three distinct topological domains.Semantic proximity Read ↗

Modeling the effects of prl mutations on the Escherichia coli SecY complex.Semantic proximity Read ↗

Mapping an interface of SecY (PrlA) and SecE (PrlG) by using synthetic phenotypes and in vivo cross-linking.Semantic proximity Read ↗

The sec and prl genes of Escherichia coli.Semantic proximity Read ↗

prl mutations in the Escherichia coli secG gene.Semantic proximity Read ↗

One of three transmembrane stretches is sufficient for the functioning of the SecE protein, a membrane component of the E. coli secretion machinery.Semantic proximity Read ↗

The PrlA and PrlG phenotypes are caused by a loosened association among the translocase SecYEG subunits.Semantic proximity Read ↗

Interfering mutations provide in vivo evidence that Escherichia coli SecE functions in multimeric states.Semantic proximity Read ↗

A new genetic selection identifies essential residues in SecG, a component of the Escherichia coli protein export machinery.Semantic proximity Read ↗

Novel prlA alleles defective in supporting staphylokinase processing in Escherichia coli.Semantic proximity Read ↗

A double counter-selection system for the study of null alleles of essential genes in Escherichia coli.Semantic proximity Read ↗

Suppression of signal sequence defects and azide resistance in Escherichia coli commonly result from the same mutations in secA.Semantic proximity Read ↗

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