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Article

The allele-specific synthetic lethality of prlA-prlG double mutants predicts interactive domains of SecY and SecE.

The EMBO journal - 1 Mar 1995

Flower A M, Osborne R S, Silhavy T J

Abstract excerpt

The secretion of proteins from the cytoplasm of Escherichia coli requires the interaction of two integral inner membrane components, SecY and SecE. We have devised a genetic approach to probe the molecular nature of the SecY-SecE interaction. Suppressor alleles of secY and secE, termed prlA and prlG, respectively, were analyzed in pair-wise combinations for synthetic phenotypes. From a total of 115 combinations,...

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Identifiers

PMID: 7889938
DOI: 10.1002/j.1460-2075.1995.tb07070.x
PMCID: PMC398161

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

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Research trail→

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The allele-specific synthetic lethality of prlA-prlG double mutants predicts interactive domains of SecY and SecE.PMID 7889938Read ↗

PrlA suppressor mutations cluster in regions corresponding to three distinct topological domains.Semantic proximity Read ↗

Modeling the effects of prl mutations on the Escherichia coli SecY complex.Semantic proximity Read ↗

Mapping an interface of SecY (PrlA) and SecE (PrlG) by using synthetic phenotypes and in vivo cross-linking.Semantic proximity Read ↗

The sec and prl genes of Escherichia coli.Semantic proximity Read ↗

prl mutations in the Escherichia coli secG gene.Semantic proximity Read ↗

One of three transmembrane stretches is sufficient for the functioning of the SecE protein, a membrane component of the E. coli secretion machinery.Semantic proximity Read ↗

The PrlA and PrlG phenotypes are caused by a loosened association among the translocase SecYEG subunits.Semantic proximity Read ↗

Interfering mutations provide in vivo evidence that Escherichia coli SecE functions in multimeric states.Semantic proximity Read ↗

A new genetic selection identifies essential residues in SecG, a component of the Escherichia coli protein export machinery.Semantic proximity Read ↗

Novel prlA alleles defective in supporting staphylokinase processing in Escherichia coli.Semantic proximity Read ↗

A double counter-selection system for the study of null alleles of essential genes in Escherichia coli.Semantic proximity Read ↗

Suppression of signal sequence defects and azide resistance in Escherichia coli commonly result from the same mutations in secA.Semantic proximity Read ↗

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