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Article

Mapping an interface of SecY (PrlA) and SecE (PrlG) by using synthetic phenotypes and in vivo cross-linking.

Journal of bacteriology - 1 Jun 1999

Harris C R, Silhavy T J

Abstract excerpt

SecY and SecE are integral cytoplasmic membrane proteins that form an essential part of the protein translocation machinery in Escherichia coli. Sites of direct contact between these two proteins have been suggested by the allele-specific synthetic phenotypes exhibited by pairwise combinations of prlA and prlG signal sequence suppressor mutations in these genes. We have introduced cysteine residues within the...

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Identifiers

PMID: 10348856
DOI: 10.1128/JB.181.11.3438-3444.1999
PMCID: PMC93811

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

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Research trail→

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Mapping an interface of SecY (PrlA) and SecE (PrlG) by using synthetic phenotypes and in vivo cross-linking.PMID 10348856Read ↗

The allele-specific synthetic lethality of prlA-prlG double mutants predicts interactive domains of SecY and SecE.Semantic proximity Read ↗

Nearest neighbor analysis of the SecYEG complex. 1. Identification of a SecY-SecG interface.Semantic proximity Read ↗

Modeling the effects of prl mutations on the Escherichia coli SecY complex.Semantic proximity Read ↗

PrlA suppressor mutations cluster in regions corresponding to three distinct topological domains.Semantic proximity Read ↗

SecY-SecY and SecY-SecG contacts revealed by site-specific crosslinking.Semantic proximity Read ↗

Two SecG molecules present in a single protein translocation machinery are functional even after crosslinking.Semantic proximity Read ↗

Bacterial protein translocation requires only one copy of the SecY complex in vivo.Semantic proximity Read ↗

Genetic analysis of an essential cytoplasmic domain of Escherichia coli SecY based on resistance to Syd, a SecY-interacting protein.Semantic proximity Read ↗

SecY and SecA interact to allow SecA insertion and protein translocation across the Escherichia coli plasma membrane.Semantic proximity Read ↗

The sec and prl genes of Escherichia coli.Semantic proximity Read ↗

The F286Y mutation of PrlA4 tempers the signal sequence suppressor phenotype by reducing the SecA binding affinity.Semantic proximity Read ↗

The PrlA and PrlG phenotypes are caused by a loosened association among the translocase SecYEG subunits.Semantic proximity Read ↗

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